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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in lots of tumors, which leads to chromosome instability, and thus could contribute to tumorigenesis. This protein was uncovered to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a significant purpose in selling cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are already explained. Two supplemental splice variants have been reported but in-depth nucleotide sequence data isn't nonetheless offered.
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Blocking peptides are peptides that bind specially for the focus on antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.
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This guidebook outlines further doable optimization of mobile and immunoassay parameters to make sure the absolute best results are...
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This tutorial outlines even further doable optimization of mobile and immunoassay parameters to ensure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind into the epitope around the target protein. By evaluating the staining with the blocked antibody compared to the antibody on your own, one can see which staining is certain.
Goal Information Cyclin E belongs to your hugely conserved cyclin household, whose users are characterized by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation styles which contribute into the temporal coordination of each mitotic event. Cyclin E types a complex with and functions as a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and it is degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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